食品科学与工程考研，食品科学与工程考研学校排名
近日，海南大学食品科学与工程学院刘星副教授课题组在谷物中赭曲霉毒素A（OTA）的超灵敏快速检测研究上取得新进展，并在工程技术领域Top期刊Journal of Hazardous Materials（中科院1区，影响因子10.588）发表了题为“Fluonanobody-based nanosensor via fluorescence resonance energy transfer for ultrasensitive detection of ochratoxin A”的研究论文。该论文第一单位为海南大学，第一作者为2019级硕士研究生苏本超，刘星副教授为唯一通讯作者，论文合作单位包括陕西师范大学、澳门科技大学、南昌大学、美国加州大学戴维斯分校。
研究表明，OTA能抑制人类和动物蛋白合成、扰乱代谢途径、破坏钙稳态和损伤DNA，主要污染豆类、谷类、香料、咖啡、牛奶、红酒、葡萄汁和肉等多种农产品，对人类健康存在潜在风险。OTA检测通常采用色谱法，由于需要精密仪器、样品前处理繁琐等，限制了基层检测的广泛应用。
刘星副教授课题组通过综合运用免疫分析和荧光共振能量转移原理，以新型荧光纳米抗体作为识别探针和能量供体，以量子点-OTA偶联物为竞争抗原和能量受体，开发了一种基于荧光纳米抗体的荧光共振能量转移纳米传感器，成功应用于谷物中OTA的超灵敏快速检测。该纳米传感器的最低检测限为5 pg/mL，线性范围为5-5000 pg/mL，只需5分钟完成检测。该技术具有快速灵敏、经济环保、高通量检测等优点，为分析复杂的食品基质环境中超低水平的小分子污染物提供了一种新的检测方法。
项目资助
该文章获得中国国家自然科学基金（31760493, 31901800, 31701705）、海南省重点研发计划（ZDYF2020157）、海南省自然科学基金（320RC509, 2019RC119）、美国NIH-NIEHS (RIVER Award)（R35 ES030443-01）和美国NIEHS Superfund Research Program（P42 ES004699）等项目的资助。
作者简介
Fluonanobody-based nanosensor via fluorescence resonance energy transfer for ultrasensitive detection of ochratoxin A
Benchao Sua1, Zhong Zhangb1, Zhichang Suna1, Zongwen Tanga, Xiaoxia Xiea, Qi Chena, Hongmei Caoa, Xi Yuc, Yang Xud, Xing Liua*, Bruce D.Hammocke
aCollege of Food Science and Engineering, Hainan University, Haikou 570228, China
bEngineering Research Center of High Value Utilization of Western Fruit Resources and College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an, Shanxi 710119, China
cFaculty of Medicine, Macau University of Science and Technology, Taipa, Macau 999078, China
dState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China
eDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, CA 95616, USA
Abstract
Ochratoxin A (OTA) contamination in food is a serious threat to public health. There is an urgent need for development of rapid and sensitive methods for OTA detection, to minimize consumer exposure to OTA. In this study, we constructed two OTA-specific fluonanobodies (FluoNbs), with a nanobody fused at the carboxyl-terminal (SGFP-Nb) or the amino-terminal (Nb-SGFP) of superfolder green fluorescence protein. SGFP-Nb, which displayed better fluorescence performance, was selected as the tracer for OTA, to develop a FluoNb-based nanosensor (FN-Nanosens) via the fluorescence resonance energy transfer, where the SGFP-Nb served as the donor and the chemical conjugates of OTA-quantum dots served as the acceptor. After optimization, FN-Nanosens showed a limit of detection of 5 pg/mL, with a linear detection range of 5–5000 pg/mL. FN-Nanosens was found to be highly selective for OTA and showed good accuracy and repeatability in recovery experiments using cereals with various complex matrix environments. Moreover, the contents of OTA in real samples measured using FN-Nanosens correlated well with those from the liquid chromatography with tandem mass spectrometry. Therefore, this work illustrated that the FluoNb is an ideal immunosensing tool and that FN-Nanosens is reliable for rapid detection of OTA in cereals with ultrahigh sensitivity.
编辑：袁艺；责编：张睿梅
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为进一步促进动物源食品科学的发展，带动产业的技术创新，更好的保障人类身体健康和提高生活品质，北京食品科学研究院和中国食品杂志社在宁波和西宁成功召开前两届“动物源食品科学与人类健康国际研讨会”的基础上，将与郑州轻工业大学、河南农业大学、河南工业大学、河南科技学院、许昌学院于2021年11月27-28日在河南郑州共同举办“2021年动物源食品科学与人类健康国际研讨会”。欢迎相关专家、学者、企业家参加此次国际研讨会。
食品科学与工程考研(食品科学与工程考研学校排名)